By Rafael Fernandez-Botran
Immunologists besides investigators in different disciplines could usually use protocols regarding the isolation, cultures and characterization of alternative different types of leukocytes. complex equipment in mobile Immunology is a set of thoughts in an easy-to-use format.Each bankruptcy offers readers with comparable application info, a step by step description of the method, replacement concepts, pertinent references, and data approximately advertisement resources for fabrics and regents. as well as leukocytes, the authors consultant readers throughout the tactics of mobilephone tradition in addition to irritation and autoimmunity in various animal models.Covering subject matters similar to PCR and Apoptosis, this ebook will serve a consultant to usual strategies in mobile immunology whereas using either human and murine versions.
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Additional info for Advanced Methods in Cellular Immunology
Check the viability; use only cells with viability higher than 95%. 2. Add Na251CrO4 to the cells (500 µCi per 1 × 107 cells) and incubate for 60 min at 37°C with occasional shaking. 3. Wash the cells at least five times by centrifugation at 300 × g for 10 min at room temperature. Check the radioactivity in the supernatant from 2125/ch01 Page 27 Thursday, September 6, 2007 3:35 PM Chapter one: Sources 27 last centrifugation. If still significantly higher than a background, repeat the washing procedure.
2. Corrigan, C. J. and Kay, A. , T cells and eosinophils in the pathogenesis of asthma, Immunol. Today, 13, 501, 1992. 3. Frick, W. , Sedgwick, J. , and Busse, W. , The appearance of hypodense eosinophils in antigen-dependent late phase asthma, Am. Rev. Respir. , 139, 1401, 1989. 4. Sedgwick, J. , Calhoun, W. , Vrtis, R. , Bates, M. , McAllister, P. , and Busse, W. , Comparison of airway and blood eosinophil function after in vivo antigen challenge, J. , 149, 3710, 1992. 5. Sedgwick, J. , Vrtis, R.
C. and Ward, P. , Role of oxygen-derived free radicals and metabolites in leukocyte dependent inflammatory reactions, Am. J. , 107, 397, 1982. 2. Weiss, S. , Tissue destruction by neutrophils, New Eng. J. , 320, 365, 1989. 3. Glasser, L. and Fiederlein, R. , The effects of various cell separation procedures on assays of neutrophil function, Am. J. Clin. , 93, 662, 1990. 4. Clark, R. A. and Nauseef, W. , Kruisbeek, A. , Margulies, D. , Shevach, E. 1. 5. , Becker, B. , The function of neutrophils isolated by a magnetic cell separation technique is not altered in comparison to a density gradient centrifugation method, J.
Advanced Methods in Cellular Immunology by Rafael Fernandez-Botran