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Download PDF by Frank J. Dixon: Advances in Immunology, Vol. 55

By Frank J. Dixon

ISBN-10: 0120224550

ISBN-13: 9780120224555

From the studies of past Volumes "Provides unrivalled worth in either educational and monetary phrases and may be bought through demanding pressed librarians as a massive precedence to be jealously defended." -JOURNAL OF scientific MICROBIOLOGY "Advances in Immunology needs to locate itself one of the so much energetic volumes within the libraries of our universities and institutions." -SCIENCE "A provocative and scholarly overview of research." -JOURNAL OF the yankee clinical organization "Deserves an enduring position in biomedical libraries as an reduction in study and in teaching." -JOURNAL OF IMMUNOLOGIC equipment

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Extra info for Advances in Immunology, Vol. 55

Example text

In some settings, cytokines may increase SCF production. For example, Aye et al. (1992) reported that human umbilical vein endothelial cells express SCF and c-kit mRNA and that the expression of SCF but not c-kit mRNA was increased by the addition of either monocyte supernatants, IL-lP, or thrombin to the culture medium. Finally, in a preliminary report, Linenberger et al. (1991) found that hydrocortisone moderately increased the level of SCF mRNA in bone marrow stromal cells from normal donors as well as from patients with Dianiond-Blackfan anemia.

SCF alone supported the proliferation of small myeloid colonies from the B220- populations. However, when both factors were combined, there was a substantial increase in the size of colonies produced from either population, and all of the progeny were B220+. Similar experiments were performed by Billips et al. (1992),who used three cycles of panning, and Funk et al. (1993),who used cell-sorting techniques, to purify the B220+ and B220- populations from normal mouse bone marrow cells. When these highly purified cells were used, no stimulation of B lymphopoiesis by IL-7 plus SCF from B220- pre-B cells was observed.

The third functionally important interval, between -81 and - 105 bp, contains conserved potential binding sites for AP-2 and S p l and two contiguous GA-rich elements (Fig. The four conserved GA-rich elements in the c-kit promoter (Fig. 6, boxes I-IV) share a core consensus sequence (G/A-G-G-A-G-G/A) also found in a noncanonical, functional PU. 1binding site is found further upstream in the c-kit promoter (Fig. 6),but this site is not well conserved and maps outside the region that is necessary for maximal promoter activity in transfected mouse mast cells or HEL cells.

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Advances in Immunology, Vol. 55 by Frank J. Dixon

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