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Advances in New Technology for Targeted Modification of - download pdf or read online

By Feng Zhang, Holger Puchta, James G. Thomson

ISBN-10: 1493925555

ISBN-13: 9781493925551

ISBN-10: 1493925563

ISBN-13: 9781493925568

Over the previous 50 years, biotechnology has been the most important driver for expanding crop productiveness. quite, advances in plant genetic engineering applied sciences have spread out sizeable new possibilities for plant researchers and breeders to create new crop kinds with fascinating qualities. fresh improvement of unique genome amendment equipment, reminiscent of distinctive gene knock-out/knock-in and specific gene substitute, strikes genetic engineering to a different point and gives much more potentials for making improvements to crop construction. The paintings offers an outline of the newest advances on specified genomic engineering applied sciences in vegetation. issues contain recombinase and engineered nucleases-mediated unique amendment, negative/positive selection-based homologous recombination and oligo nucleotide-mediated recombination. eventually, demanding situations and affects of the hot applied sciences on current rules for genetic amendment organisms (GMOs) could be discussed.

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Download PDF by Feng Zhang, Holger Puchta, James G. Thomson: Advances in New Technology for Targeted Modification of

During the last 50 years, biotechnology has been the most important motive force for expanding crop productiveness. relatively, advances in plant genetic engineering applied sciences have unfolded great new possibilities for plant researchers and breeders to create new crop forms with fascinating qualities. fresh improvement of distinctive genome amendment tools, resembling particular gene knock-out/knock-in and specific gene substitute, strikes genetic engineering to a different point and provides much more potentials for making improvements to crop creation.

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Additional resources for Advances in New Technology for Targeted Modification of Plant Genomes

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2002; Segal et al. 2003; Mandell et al. 2006). Although straightforward, modular assembly generally has a low successful rate in generating active ZFNs due to low activities and/or high toxicities (Ramirez et al. 2008; Cornu et al. 2008). With a prescreen for enriching fingers that were often found in functional ZFNs, a higher successful rate (23 %) was reported for modular assembly (Kim et al. 2011). More recently, Bhakta et al. developed an extended modular assembly method with a much higher successful rate (71 %) when variant lengths of zinc finger arrays (ZFAs) were tested (Bhakta et al.

Choulika A, Perrin A, Dujon B, Nicolas JF (1995) Induction of homologous recombination in mammalian chromosomes by using the I-SceI system of Saccharomyces cerevisiae. Mol Cell Biol 15(4):1968–1973 Daboussi F, Zaslavskiy M, Poirot L et al (2012) Chromosomal context and epigenetic mechanisms control the efficacy of genome editing by rare-cutting designer endonucleases. Nucleic Acids Res 40(13):6367–6379 Delacote F, Perez C, Guyot V et al (2011) Identification of genes regulating gene targeting by a high-throughput screening approach.

Nucleic Acids Res 39(14):6124–6136 Grosse S, Huot N, Mahiet C et al (2011) Meganuclease-mediated Inhibition of HSV1 Infection in Cultured Cells. Mol Ther 19(4):694–702 Heath PJ, Stephens KM, Monnat RJ Jr, Stoddard BL (1997) The structure of I-Crel, a group I intron-encoded homing endonuclease. Nat Struct Biol 4(6):468–476 2 Engineering Meganuclease for Precise Plant Genome Modification 37 Hu D, Crist M, Duan X, Gimble FS (1999) Mapping of a DNA binding region of the PI-SceI homing endonuclease by affinity cleavage and alanine-scanning mutagenesis.

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Advances in New Technology for Targeted Modification of Plant Genomes by Feng Zhang, Holger Puchta, James G. Thomson


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